The Automated Cell Deposition Unit (ACDU) option is available for sorting directly into multi-well plates or onto microscope slides. The Fusion offers two- and four-way bulk sorting devices for 1.5mL microfuge tubes, 12 x 75mm 5mL tubes or 15mL conical tubes. Three sizes of nozzles (70, 85 and 100-micron) are offered to accomodate a wide range of particle sizes. The Fusion has been verified to meet personnel and product protection standards for a Class II, Type A2 biosafety cabinet as specified by the National Sanitation Foundation International Standard 49. The Fusion sits inside a custom designed biosafety cabinet to address the concerns of biological safety issues and to protect operators from potential accidental exposure from biological samples. The BD Fusion is a state-of-the-art cell sorter equipped with five dedicated lasers (355nm, 405nm, 488nm, 561nm, and 637nm) for excitation and 20 parameters for detection, which includes 2 light scatter parameters (forward and side scatter) and 18 fluorescence parameters. To schedule Assisted Use or Training please contact Richard Salinas.įees: Trained user: $38/hour. Assisted use: $98/hour Instrument Access: Assisted use and training is available. Sample analysis can also be done using Flowjo or FCS Express (both available to use in NMS 2.114).
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While users running multi-well plates will use IntelliCyt ForeCyt iDM Server Edition software (v3.1). Users running samples in tubes will use BD Accuri C6 software (v1.0) for acquisition and analysis of samples.
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The Accuri will accept standard 5mL test tubes or 1.5mL microfuge tubes.įor High-Throughput Screening, the optional IntelliCyt HyperCyt Autosampler plate reader can be easily connected to the Accuri cytometer and is capable of screening a 96 multi-well plate in 5 minutes or a 384 multi-well plate in 11 minutes. After data is collected, the BD Accuri C6 software Zoom function allows for visualization of data at any scale, which allows for precise placement of gates and regions. Gating strategies and fluorescence compensation values can be set before, during, or after data collection. This results in a standardized fluorescence performance so that users do not need to adjust detector voltages.ĭata is collected over 7-decade dynamic range (16 million channels of digital data), making all data available to users as needed. The laser and optical alignments are set and locked down during the manufacturing of the instrument. The Accuri is equipped with two 20mW lasers (488nm and 552nm) and 6 parameters for detection, which include 2 light scatter parameters (forward scatter and side scatter) and 4 parameters for acquiring fluorescence. To schedule Assisted Use or Training please contact Richard Salinas.įees: Trained user: $38/hour. Assisted use: $98/hour BD Accuri SORP Flow Cytometer Instrument Access: Assisted use and training is available. Instrument Configuration: Fortessa Configįluorescence Spectrum Analyzer (BD Biosciences) The Fortessa only accepts 5mL polystyrene tubes, or 96- and 384-multi-well plates if using the HTS unit. Sample analysis can also be done using Flowjo or FCS Express (both available to use in NMS2.114). Sample acquisition and analysis is done using FACSDiva v6.1.3.
In addition, this system is also equipped with the FACSFlow Supply System to allow larger volume tanks for sheath and waste, as well as improved fluidic stability. This instrument can be connected to the optional High Throughput Sampler (HTS) unit to accommodate automated specimen handling for 96- and 384-well plates. The Fortessa is equipped with four lasers (405nm, 488nm, 561nm and 635nm) and 15 parameters for detection, which includes 2 light scatter parameters (forward and side scatter) and 13 fluorescence parameters.
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